品牌:The Native Antigen Company
貨號(hào):PAB21462-25
品名:Rabbit Anti-Borrelia burgdorferi sensu stricto (B31) OspB Antibody
規(guī)格:25ug
Rabbit Anti-Borrelia burgdorferi OspB antibody is a polyclonal recognising Borrelia burgdorferi sensu stricto. The antibody is suitable for use in ELISA and Western blotting applications.
商品詳情參考文獻(xiàn)相關(guān)資料
產(chǎn)品介紹
品名
Rabbit Anti-Borrelia burgdorferi sensu stricto (B31) OspB Antibody
貨號(hào)
PAB21462-25、PAB21462-100
規(guī)格
25 ug 、100ug
類型
Polyclonal
同種型
Rabbit IgG
緩沖液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
防腐劑
0.01% (w/v) Sodium Azide
免疫原
Recombinant MBP tagged B. burgdorferi OspB protein
純化
Protein-A purified and cross-adsorbed against MBP from monospecific
antiserum by chromatography
特異性
This antibody is specific for Borrelia burgdorferi OspB protein. A BLAST
analysis was used to suggest cross-reactivity with OspB from B. burgdorferi,
afzelii, spielmanii, and garinii sources based on 100% homology with the
immunizing sequence, and with B. valaisiana based on 99% homology. Crossreactivity with OspB from other sources has not been determined.
應(yīng)用
ELISA (1:13,000), WB (1:1000)
貯存
Store vial at -20° C or below prior to opening. To minimize loss of volume, dilute
1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap,
mix thoroughly and briefly centrifuge to collect the volume at the bottom of
the vial. Use this intermediate dilution when calculating final dilutions as
recommended above. Store the vial at -20°C or below after dilution. Avoid
cycles of freezing and thawing.