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Baboon, Guinea Pig, Cat, Rabbit, Hamster, Horse

2°C to 8°C  (≤ 30 days)

For long-term storage (> 30 days), aliquot and store at ≤ –20°C. Avoid repeated freeze-thaw.

For Research Use Only. Not for use in diagnostic procedures

Highly purified human C7 was isolated from normal serum and used to immunize goats. The anti-human C7 polyclonal antisera was tested against normal human plasma by double immunodiffusion, one-dimensional immunoelectrophoresis, quantitative radial immunodiffusion, and quantitative rocket immunoelectrophoresis. The antiserum was determined to be monospecific for C7 at varying concentrations.

Quidel Polyclonal Antiserum to Human C7 Protein

C7 is a glycoprotein which is present in normal human serum/plasma at approximately 55 μg/mL. Primarily synthesized in the liver, C7 is a single-chain protein with a molecular weight of approximately 95 kD. This complement protein is key for the continued formation of the SC5b-9 complex after C5b binds to C6

With activation of the classical or alternative pathways of the complement system, C5 convertase cleaves the C5 chain into C5a and C5b. C5b remains bound to the convertase and will combine with C6 to form a C5b,6 complex. This complex normally interacts with C7 to form C5b,6,7 that becomes partially embedded in the target cell outer membrane.

Applications of the C7 polyclonal antisera have been evaluated by various research facilities, and include, Western Blot, 1 Radial Immunodiffusion, 2 Immunofluorescence3 and Immunoblot. 4