Product Details
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Product Name
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Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody [RM119]
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Product Description
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Biotinylated Rabbit monoclonal to Human IgG3
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Catalog No.
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31-1021-02
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Clone Name
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RM119
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Product Data Sheet
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Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119
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Specificity
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RM119 reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
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Immunogen
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Human IgG3
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Application
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Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
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Species Reactivity
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Human
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Conjugate
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Biotin
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Item Size
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50 ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
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Keywords
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Product Images
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Immunohistochemistry of Human Lymphoid Tissue using
Anti-Human IgG3 antibody RM119.
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Sandwich ELISA using RM119 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an HRP conjugated streptavidin.
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Sandwich ELISA using RM119 as the capture antibody
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an HRP conjugated streptavidin.
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ELISA of human immunoglobulins shows RM119
reacts only to human IgG3. No cross reactivity
with Human IgG1, IgG2, IgG4, IgE, IgD, IgA,
mouse IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM119 was used
as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.
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A titer ELISA using RM119. The plate was coated
with different amounts of human IgG3. A serial dilution
of RM124 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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