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Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM181; H3K79me2, Histone H3 (dimethyl K79)

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Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM181; H3K79me2, Histone H3 (dimethyl K79)

品牌：RevMAb

貨號：31-1065-00

規(guī)格：100 ug

貨期：電詢

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Product Details
Product Name	Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [RM181]
Product Description	Rabbit monoclonal to Dimethylated Histone H3 Lysine 79, H3K79me2, Histone H3 (dimethyl K79)
Catalog No.	31-1065-00
Clone Name	RM181
Product Data Sheet	Anti-Dimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody Data Sheet RM181
Specificity	RM181 reacts to Histone H3 dimethylated at Lysine 79 (K79me2). ). Very slightly cross-reacts with monomethylated Lysine 79 (K79me1) at high concentration. No cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.
Immunogen	A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys79).
Application	Western Blot (WB) Chromatin IP (ChIP) Immunohistochemistry (IHC) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.25 ug/mL – 1 ug/mL; IHC: 0.1 ug/mL – 1 ug/mL; ChIP: 2 ug/mL- 10 ug.mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
	RM181 specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.
	Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM181 at 0.25 ug/mL, showed a band of histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.
	ChIP performed on HeLa cells using H3K79me2 antibody (RM181, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
	Immunohistochemistry staining of HepG2 cells usingAnti-Dimethyl-Histone H3 (Lys79) antibody, RM181.