Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM137
Specificity
RM137 reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1), dimethylated Lysine 4 (K4me3), or other methylations in histone H3.
Immunogen
A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4).
Application
Western Blot
ELISA
Multiplex
Species Reactivity
Human
Vertebrates
Conjugate
None
Intended Use
Research Use Only
.
Properties
Form
Liquid
Storage Instructions
Store at -20.0°C
Stability
Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size
100 ug
Concentration
1.0 mg/mL
Purity
Protein A affinity purified from an animal origin–free culture supernatant
A Peptide dotblot shows RM137 reacts only to Histone H3 trimethyl-Lysine 4 (K4me3). No cross reactivity with nonmodified Lysine 4 (H3N1-19), monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me2).
RM137 specifically reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with other methylated lysines in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM137 at 0.5 ug/mL, showed a band of histone H3 tri- methylated at Lysine 4 (K4me3) in HeLa cells.