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Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM137; H3K4me3, Histone H3 (trimethyl K4)
品牌:RevMAb
貨號(hào):31-1039-00
規(guī)格:100 ug
貨期:電詢(xún)
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Product Details
Product Name Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM137]
Product Description Rabbit monoclonal to Trimethylated Histone H3 Lysine 4, H3K4me3, Histone H3 (trimethyl K4)
Catalog No. 31-1039-00
Clone Name RM137
Product Data Sheet Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM137
Specificity RM137 reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1), dimethylated Lysine 4 (K4me3), or other methylations in histone H3.
Immunogen A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4).
Application Western Blot
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.2 ug/mL – 1 ug/mL;
ELISA: 0.1 ug/mL – 0.5 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
.
Product Images
A

A Peptide dotblot shows RM137 reacts only to
Histone H3 trimethyl-Lysine 4 (K4me3). No cross
reactivity with nonmodified Lysine 4 (H3N1-19),
monomethylated Lysine 4 (K4me1) or dimethylated
Lysine 4 (K4me2).

RM137

RM137 specifically reacts to Histone H3 trimethylated
at Lysine 4 (K4me3). No cross reactivity with other
methylated lysines in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1) and
acid extracts of HeLa cells (2), using RM137 at
0.5 ug/mL, showed a band of histone H3 tri-
methylated at Lysine 4 (K4me3) in HeLa cells.


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