Product Details
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Product Name
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Anti-Human IgG3 Rabbit Monoclonal Antibody [RM119]
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Product Description
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Rabbit monoclonal to Human IgG3
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Catalog No.
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31-1021-00
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Clone Name
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RM119
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Product Data Sheet
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Anti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119
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Specificity
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RM119 reacts to the heavy chain of human IgG3, RM119 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG4), and shows no cross reactivity IgM, IgA, IgD, IgE. RM119 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
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Immunogen
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Human IgG3
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Application
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Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
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Species Reactivity
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Human
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Conjugate
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None
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Intended Use
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Research Use Only
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Properties
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Form
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Liquid
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Storage Instructions
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Store at -20.0°C
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Stability
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Stable for 1 Year at -20.0°C from date of receipt
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Storage Buffer
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50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
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Volume/Item Size
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100ug
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Concentration
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1.0 mg/mL
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Purity
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Protein A affinity purified from an animal origin–free culture supernatant
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Clone Type
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Monoclonal
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Isotype
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Rabbit IgG
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Usage
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ELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
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Keywords
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Product Images
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Detection of human IgG3 in monkey serum.
Sandwich ELISA using RM119 as the capture
antibody, and biotinylated anti-human light
chains ( κ+ λ) antibody RM129 as the detection
antibody, followed by a HRP conjugated streptavidin.
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Sandwich ELISA using RM119 as the capture antibody
(100ng/well), and biotinylated anti-human light chains ( κ+ λ)
antibody RM129 as the detection antibody,
followed
by an HRP conjugated streptavidin.
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ELISA showing RM119 reacts only to human IgG3,
and not to any other IgG subclasses (IgG1, IgG2,
or IgG4), and no cross reactivity to IgM, IgA, IgD,
IgE, mouse IgG, rat IgG, or goat IgG.
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A titer ELISA using RM119. The plate was coated
with different amounts of human IgG3. A serial dilution
of RM119 was used as the primary antibody, followed
by an alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.
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Immunohistochemical staining of formalin fixed
and paraffin embedded human lymphoid tissue
section using anti-human IgG3 rabbit monoclonal
antibody (Clone RM119).
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ELISA showing RM119 does not react to monkey
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM119 and a monkey IgG binding
antibody (positive control) was used as the detection
antibody.
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Immunohistochemical staining of FFPE Tonsil tissue
sections using Anti-Human IgG3 antibody RM119
at a 1:2000 dilution.
Image courtesy of Shaomin Hu, MD, PhD,
Department of Anatomic and Clinical Pathology.
Montefiore Medical Center
Albert Einstein College of Medicine.
Bronx, New York, USA
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