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Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175; H3K27me3, Histone H3 (trimethyl K27); ChIP Validated

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Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175; H3K27me3, Histone H3 (trimethyl K27); ChIP Validated

品牌：RevMAb

貨號(hào)：31-1062-00

規(guī)格：100 ug

貨期：電詢

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Product Details
Product Name	Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [RM175]
Product Description	Rabbit monoclonal to Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27)
Catalog No.	31-1062-00
Clone Name	RM175
Product Data Sheet	Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody Data Sheet RM175
Specificity	RM175 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3.
Immunogen	A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27).
Application	Western Blot (WB) Immunohistochemistry (IHC) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 1 ug/mL – 2 ug/mL; IHC: 0.5 ug/mL – 2 ug/mL; ELISA: 0.5ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
	RM175 specifically reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2),or other methylations in Histone H3.
	Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM175 at 1 ug/mL, showed a band of histone H3 trimethyl-ated at Lysine 27 (K27me3) in HeLa cells.
	Immunohistochemistry staining of HepG2 cells usingAnti-Trimethyl-Histone H3 (Lys27) antibody, RM175.