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Anti-Histone H3.3 G34R Rabbit Monoclonal Antibody, Clone RM240; Histone H3.3 G34R Mutant

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Anti-Histone H3.3 G34R Rabbit Monoclonal Antibody, Clone RM240; Histone H3.3 G34R Mutant

品牌：RevMAb

貨號(hào)：31-1120-00

規(guī)格：50 ug

貨期：電詢

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Product Details
Product Name	Anti-Histone H3.3 G34R Rabbit Monoclonal Antibody [RM240]
Product Description	Rabbit monoclonal to Histone H3.3 G34R; Histone H3.3 G34R Mutant
Catalog No.	31-1120-00
Clone Name	RM240
Product Data Sheet	Anti-Histone H3.3 G34R Mutant Rabbit Monoclonal Antibody Data Sheet RM240
Specificity	RM240 reacts to the Histone H3.3 G34R mutant. No cross reactivity with wild type Histone H3.3 .
Immunogen	A peptide corresponding to Histone H3.3 G34R mutant
Application	Western Blot ELISA Immunohistochemistry Immunocytochemistry Chromatin IP
Species Reactivity	All
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	50 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.5 ug/mL – 2 ug/mL; ELISA: 0.5 ug/mL - 2 ug/mL; Immunohistochemistry (IHC): 0.5 – 5 ug/mL; Immunocytochemistry (ICC): 0.5 – 5 ug/mL.
References for Histone H3.3 G34R Mutant antibody [RM240]	Pathania et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017)10.1016/j.ccell.2017.09.014. WB; Human. Read more (PubMed: 29107533) Yamamoto H.et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017)10.1016/j.humpath.2017.11.020 IHC; Human. Read more (PubMed: 29241742)
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Product Images
	Western Blot analysis of cell lysates prepared from 293T, transfected with a DNA construct encodingG34R mutant or wild type proteins of Histone H3.3, using anti-Histone H3.3 G34R clone RM240.
	Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNAconstruct encoding Histone H3.3 G34R mutant or wild type, stained with anti-Histone H3.3 G34R clone RM240.
	IHC of clinically validated FFPE Glioblastoma tumortissues with H3.3 G34R expression, using anti-histone H3.3 G34R rabbit monoclonal antibody, Clone RM240. Clinically validated to be Positive for H3.3 G34R Mutation. Image courtesy of Dr. Sebastian Brandner, UCL Institute of Neurology, London, United Kingdom
	IHC of clinically validated FFPE Glioblastoma tumortissues, using anti-histone H3.3 G34R rabbit monoclonal antibody, Clone RM240. Clinically validated to be Negative for H3.3 G34R Mutation. Image courtesy of Dr. Sebastian Brandner, UCL Institute of Neurology, London, United Kingdom
	ICC staining of primary human cell lines expressing WT and H3.3 G34R Mutation. Image courtesy of Dr. Nada Jabado, McGill University, Montreal, Quebec, Canada.