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5Flu-GFCPDHKAAMVLFLDSVYGIEVQDFLLHLLEVGFLP
Negative control for fluorescein DPc10 peptide.
DPc10 inactive peptide is a 36-residue peptide that differs from DPc10 (Gly2460-Pro2495, rabbit) at one position (R2475S) and represents the sequence of a known CPVT mutant RYR2 domain. This peptide binds weakly or fails to bind to the DPc10 interface and does NOT affect channel function.
DPc10 inactive in the current form is not cell permeable. The product is useful in applications that permit access to RYR2 directly (via whole cell patch, skinned cells, black lipid membrane, vesicles, or upon delivery to cytoplasm).
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Fluorescein DPc10 Inactive Peptide
5-Carboxyfluorescein
Acid
4093.046
Analysis carried out using a 100? 4.6 x 150mm column, gradient from 10%-90% acetonitrile, in 15 minutes. 60 degC. Purity determined as 97%
Molecular ion predicted: 4393.046 . Observed: 4392.6 [M+3H]
Yellow powdered solid
DMSO
Store as supplied at -20°C for up to 1 year. Store desiccated. Make fresh solutions prior to use.
The product was purified in acetonitrile and water containing 0.1% trifluoroacetic acid (TFA) prior to lyophilisation. The product will therefore be present as its trifluoroacetic acid salt.
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