Strain EVB101, an electro-competent E. coli B strain (hsdR, lon11, sulA1), contains pBirAcm , an IPTG inducible plasmid containing the BirA gene engineered into pACYC184. It is compatible with most cloning vectors and is maintained with chloramphenicol (10 μg/ml). This strain is recommended for protein expression because of its robust growth and the absence of the OmpT and Lon proteases.

At the time of induction, Biotin should be added at a concentration of 50 μM. The pAN and pAC vectors containing the AviTag^TM require ampicillin (100 μg/ml).