Increased serum/plasma concentrations of Neuropeptide Y (NPY) have been found in patients with neuroblastoma and pediatric B-cell precursor leukemia.
Elevated levels of NPY have been found in serum/plasma from patients with phaeochromocytoma tumours. Assay of NPY in serum/plasma may serve as valuable tool in the diagnosis of neuroblastoma and pediatric B-cell precursor leukemia and phaeochromocytoma.
The NPY EURIA is a highly sensitive and specific radioimmunoassay for the determination of NPY in human serum/plasma by direct assay without extraction.
Intended use
The intended use of these reagents is for assay of NPY in human serum/plasma by direct assay without extraction.
For professional use within a laboratory.
This product is also available as research use only. Product code: RB 317 RUO
For complete product description, claims and use, please refer to the Instructions For Use. Contact your local representative for availability in your country.
Kit components and storage of reagents
The reagents provided in each kit are sufficient for 100 tubes.
Composition of the reagent kit
1. Anti-NPY (Reagent A)
Rabbit antiserum raised against NPY conjugated to bovine thyroglobulin. Lyophilized in
2.0 mL 0.5 M phosphate buffer, pH 7.4, with 2.5% human serum albumin, 2.5% EDTA, 1.0% Triton X-100, 0.5% sodium azide and 5000 KIU Trasylola per mL. For 100 tubes. Reconstitution in 22 mL distilled water.
Colour: Yellow.
2. 125I-NPY (Reagent B)
Contains 28 KBq or 0.75 μCi at reference date. Synthetic, human NPY is iodinated according to Bolton-Hunter. The monoiodinated form is purified by HPLC. Specific activity: 1700-2100 μCi/nmol (62-77 MBq/nmol). Lyophilized in 2.5 mL 0.5 M phosphate buffer, pH 7.4, with 2.5% human serum albumin, 2.5% EDTA, 1.0% Tween 80, 0.5% sodium azide and 5000 KIU Trasylola per mL. Reconstitution in 25 mL distilled water.
Colour: Blue.
3. Double antibody solid phase (Reagent C)
11.0 mL suspension. Anti-rabbit-Ig coupled to cellulose particles.
4. Standard diluent (Reagent D)
10.0 mL NPY-free human serum, lyophilized. Contains 500 KIU Trasylola per mL. Reconstitution in 10.0 mL distilled water. For preparation of NPY working standards.
5. NPY standard 3000 pmol/L (Reagent E)
Lyophilized. 2.00 mL standard. Concentration: 3000 pmol/L after reconstitution. The standard is produced from synthetic, human NPY. Lyophilized in 0.05 M phosphate buffer, pH 7.4, with 0.25% human serum albumin, 0.25% EDTA, 0.1% Triton X-100, 0.05% sodium azide and 500 KIU Trasylola per mL. For preparation of NPY working standards.
6. Assay buffer (Reagent F)
5.0 mL 0.05 M phosphate buffer, pH 7.4, with 0.25% human serum albumin, 0.25% EDTA, 0.1% Triton X-100, 0.05% sodium azide and 500 KIU Trasylola per mL.
To be used instead of antiserum in non-specific binding test tubes.
7. Controls (Reagent G-H)
Lyophilized serum controls with low and high concentration of NPY. 2.00 mL of each control after reconstitution. The NPY concentrations are given on the labels of the vials.
Contains 0.05% sodium azide.
Reagent preparation and storage
Store all reagents at 2-8° C before reconstitution and use. The water used for reconstitution of lyophilized reagents should be distilled in an all-glass apparatus or be of corresponding purity. Dissolve the contents in a vial by gentle inversion and avoid foaming. The stability of the reagents is found on the labels of the vials. For lyophilized reagents the expiry dates are valid for the unreconstituted reagents. The reconstituted reagents are stable for 10 weeks (no longer than to the expiry date) if stored properly.
