Tissue homogenates, cell Lysates and other biological fluids.
Assay Time
3 hours
UniProt
Q60767
Gene ID
17076
Gene Name
Lymphocyte Antigen 75
Gene Aliases
CD205; LY75; CLEC13B; DEC-205; GP200-MR6; C-type lectin domain family 13 member B
Application
ELISA
Assay Type
Sandwich
Detection Method
Colorimetric
Shipping Condition
Blue ice
Storage Instructions
-20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date
8 months
Specificity
This assay has high sensitivity and excellent specificity for detection of Lymphocyte Antigen 75 (LY75). No significant cross-reactivity or interference between Lymphocyte Antigen 75 (LY75) and analogues was observed.
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Antigen 75 (LY75). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Antigen 75 (LY75). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Antigen 75 (LY75), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Antigen 75 (LY75) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphocyte Antigen 75 (LY75) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and hi
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions
For research use only. Not for diagnostic procedures.
Quality Systems
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area
Signal transduction;CD & Adhesion molecule;Infection immunity;