Anti-Pregnancy associated plasma protein A (human, PAPP-A)
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Cat. No.
ABS 006-24
Product name
Anti-Pregnancy associated plasma protein A (human, PAPP-A)
Description
Mouse monoclonal antibody
Specificity
ABS 006-24 is specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A .
Immunogen
Purified human PAPP-A
Application
ELISA, WB, IHC
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass
IgG1/k
Gene ID
5069
Clone number
PAPP A 1-24
Epitope
The epitope of ABS 006-24 is different from BTE 004-09 and ABS 006-01. The epitope is located on the PAPP-A subunit of the heterotetramer.
Content
Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8oC without exposure to light. No precautions necessary during handling.
Target
Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (trisomy 21).
Application 1
ELISA
BTE 004-09 as coating antibody and biotinylated ABS 006-24 as detection antibody is a good combination for sandwich ELISA.
Application 2
WB
ABS 006-24 only detecs unreduced PAPP-A in Western Blotting.
Application 3
IHC
ABS 006-24 gives the strongest singal in IHC compared to ABS 006-01 and BTE 004-09.
?
Immunohistochemical staining of a formalin-fixed, paraffin-embedded placental tissue with ABS 006-24