Human Monoclonal Antibody, Biotinylated
This product is purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B.Must be handled as potentially infectious as all human material.The purity of the human IgE is > 90%, tested by SDS-PAGE.As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The human IgE is purified by Protein L chromatography. The remaining contaminants are mainly components from Foetal Bovine Serum.
Non-immune
IgE/k
100 μl, 1mg/mL
Quantifying Assay This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.
IHC This antibody can be used in immunochemistry
Investigation of Aspergillus oryzae involvement in allergic bronchopulmonary aspergillosis (ABPA) 4. Yamaki K and Yoshino S (2011) Aspergillus oryzae Lectin Induces Anaphylactoid Oedema and Mast Cell Activation Through its Interaction With Fucose of Mast Cell–Bound Non-specific IgE. Scandinavian Journal of Immunology 74:445–453.
Used as a control for IgE in skin graft on mice 5. Fairley JA, Burnett CT, Fu CL, Larson DL, Fleming MG, Giudice GJ (2007) A pathogenic role for IgE in autoimmunity: Bullous pemphigoid IgE reproduces the early phase of lesion development in human skin grafted to nu/nu mice. J Invest Dermatol 127:2605-2611.
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