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Concentration
20u/μl 
5 ' . . . C↓C AT G G . . . 3 '
3 ' . . . G G TA C↑C . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol.

Unit Definition
20u(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37?C in 20μl or 50μl of assay buffer.

Thermal Inactivation
65?C for 20 minutes.

Quality Control Assays
Ligation/ Recutting Assay
After 20-fold overdigestion with Bsp19 I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of Bsp19 I for 16 hours at 37?C.

Ordering Information

Downloads
Manual
Bsp19 I (Nco I)