Long trebler phosphoramidite is a branching reagent for oligonucleotide synthesis allowing to synthesize branched DNA structures using standard DNA synthesizer. After the condensation of the trebler, three DNA branches begin to grow simultaneously with each step of the synthesis. Deblock of this construct gives rise to DNA containing branching point. One arm (stem) is attached to branch point with its 5'-end, and other arms (branches) are attached via 3'-end. Reverse amidites can be used to prepare constructs with different branch orientations. Repetitive condensations of trebler results in formation of DNA dendrimers. Trebler amidite can be used to attach several modifier amidites to 5'-end of oligonucleotide - for example, three biotin residues can be attached at once. This amidite does not require any special handling. Recommended coupling time is 5 minutes. No changes to deblock conditions are required.
General properties
Appearance:
viscous yellowish oil
Molecular weight:
1475.78
Molecular formula:
C89H107N2O15P
Solubility:
soluble in acetonitrile, dichloromethane
Quality control:
NMR 1H (95%), 13C, 31P, HPLC
Storage conditions:
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:
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