This high load, 500 ? controlled pore glass solid support is designed for the synthesis of oligonucleotides containing up to 50 bases with a 3'-amino group. Amino group is protected with trifluoroacetyl (TFA) protection which is easily removed under standard deblock conditions. The reagent is based on hydroxyprolinol core - a universal non-nucleoside structure that is naturally 100% chiral (no isomers formed upon condensation), and stable to all conditions of oligonucleotide synthesis and deblock.
General properties
Appearance:
off-white beads
Quality control:
NMR 1H and HPLC (95%) of bound reagent, loading measurement
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
CPG details
Pore size, ?:
500
Typical loading, umol/g:
70?90
Coupling conditions:
standard coupling, identical to normal nucleobases
Cleavage conditions:
ammonia, 2 h at room temperature
Deprotection conditions:
identical to protected nucleobases
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