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Cyanine5 NHS ester
品牌:
貨號(hào):13020
規(guī)格:
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訂購(gòu)貨號(hào) 產(chǎn)品名稱及規(guī)格 價(jià)格(¥) 13020 Cyanine5 NHS ester, 1 mg 1430.00 23020 Cyanine5 NHS ester, 5 mg 2730.00 43020 Cyanine5 NHS ester, 25 mg 5330.00 53020 Cyanine5 NHS ester, 50 mg 9035.00 63020 Cyanine5 NHS ester, 100 mg 15470.00

商品詳情 參考文獻(xiàn) 相關(guān)資料

Cyanine5 NHS ester

Cyanine5 NHS ester

詳細(xì)描述

 

訂購(gòu)貨號(hào) 產(chǎn)品名稱及規(guī)格 價(jià)格(¥)
13020 Cyanine5 NHS ester, 1 mg 1430.00
23020 Cyanine5 NHS ester, 5 mg 2730.00
43020 Cyanine5 NHS ester, 25 mg 5330.00
53020 Cyanine5 NHS ester, 50 mg 9035.00
63020 Cyanine5 NHS ester, 100 mg 15470.00

 

 


在過(guò)去的幾年里,CY5熒光團(tuán)已經(jīng)成為生命科學(xué)研究和診斷領(lǐng)域非常流行的分子標(biāo)記物。這些熒光素的*強(qiáng)發(fā)射光為紅光,在這個(gè)范圍大多數(shù)CCD檢測(cè)器具有*大檢測(cè)靈敏度,而且是生物物質(zhì)低背景區(qū)。染料的顏色是非常強(qiáng)烈的,因此在凝膠電泳中低至1nmol的量都能被肉眼觀測(cè)到。CY5 NHS ester是用于標(biāo)記多肽、蛋白和寡核苷酸的氨基基團(tuán)的活性染料。在標(biāo)記反應(yīng)中該染料需要少量的有機(jī)共溶劑(如DMF,DMSO)(請(qǐng)參照我們的推薦手冊(cè)以獲得更多幫助信息)。對(duì)于可溶性蛋白、各種各樣的多肽和寡核苷酸而言,這種染料是非常理想而且成本低廉的。該染料在有機(jī)溶劑對(duì)小分子物質(zhì)的標(biāo)記也是非常有效的。對(duì)于更多的精細(xì)目標(biāo)物,如易降解蛋白,如果DMF或DMSO會(huì)對(duì)其有影響,可以考慮water-soluble Cy5 NHS ester(水溶性Cy5 NHS ester染料),因?yàn)樗鼈儾恍枰袡C(jī)溶劑助溶,而且具有和熒光染料類似的性質(zhì)。

 

CY5熒光團(tuán)可以與很多的儀器如熒光顯微鏡、成像儀、掃描儀、熒光分析儀等相兼容。許多的CY5衍生物——CY5 NHS ester可以替代Alexa Fluor 647和 DyLight 649等活化酯(activated esters)。

 

 

圖片關(guān)鍵詞

 

推薦手冊(cè)

氨基分子的NHS酯標(biāo)記

 


Cy® is a trademark of GE Healthcare.


General properties

 

Appearance:

dark blue powder

Molecular weight:

616.19

Molecular formula:

C36H42ClN3O4

CAS number:

1032678-42-4, 350686-88-3

Solubility:

good in polar (DMSO, DMF) and chlorinated (DCM, chlroform) organic solvents, low solubility in water

Quality control:

NMR 1H (95%) and 13C, TLC, functional testing

Storage conditions:

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

MSDS:

Download

Spectral properties

 

Excitation maximum, nm:

646

Extinction coefficient at excitation maximum, Lmol-1cm-1:

250000

Emission maximum, nm:

662

Fluorescence quantum yield:

0.2

 


Product citations

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  • Albertazzi, L.; Martinez-Veracoechea, F.J.; Leenders, C.M.A.; Voets, I.K.; Frenkel, D.; Meijer, E.W.Spatiotemporal control and superselectivity in supramolecular polymers using multivalency.Proceedings of the National Academy of Sciences2013, 110(30), 12203-12208. doi:10.1073/pnas.1303109110
  • Cheng, M.-C.; Leske, A.T.; Matsuoka, T.; Kim, B.C.; Lee, J.; Burns, M.A.; Takayama, S.; Biteen, J.S. Super-Resolution Imaging of PDMS Nanochannels by Single-Molecule Micelle-Assisted Blink Microscopy.The Journal of Physical Chemistry B2013, 117(16), 4406-4411. doi: 10.1021/jp307635v
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  • He, H.; Chen, S.; Zhou, J.; Dou, Y.; Song, L.; Che, L.; Zhou, X.; Chen, X.; Jia, Y.; Zhang, J. et al.Cyclodextrin-derived pH-responsive nanoparticles for delivery of paclitaxel. Biomaterials2013, 34(21), 5344-5358. doi: 10.1016/j.biomaterials.2013.03.068
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  1. Chiang, W.-L.; Lin, T.-T.; Sureshbabu, R.; Chia, W.-T.; Hsiao, H.-C.; Liu, H.-Y.; Yang, C.-M.; Sung, H.-W. A rapid drug release system with a NIR light-activated molecular switch for dual-modality photothermal/antibiotic treatments of subcutaneous abscesses. Journal of Controlled Release,2015, 199, 53–62. doi: 10.1016/j.jconrel.2014.12.011
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